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sigL [2017-04-12 09:01:58]
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sigL [2017-04-12 09:01:58]

sigma factor of the RNA polymerase, Sigma-54, Sigma L
locus
BSU34200
pI
7.79
mw
49.54 kDa
protein length
436 aa Sequence Blast
gene length
1308 bp Sequence Blast
function
utilization of arginin, acetoin and fructose, required for cold adaptation
product
RNA polymerase sigma-54 factor (sigma-L)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon]

    • Gene

    Coordinates
    3,512,498 -> 3,513,808

    Phenotypes of a mutant

  • The mutant is cold-sensitive and unable to use arginine as a single carbon source. [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]

    • The protein

    Catalyzed reaction/ biological activity

  • Binding to promoters of the -12, -24 type

    • Protein family

  • sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family

    • [SW|Domains]

  • DNA binding domain (H-T-H motif) (324–343)
  • pron box domain (413–421)
  • 3 x Compositional bias domain (6–21),(32–53),(112–136)

    • Structure

  • [PDB|5BYH] (''E. coli'' [SW|RNA polymerase] containing Sigma-54) [Pubmed|26293966]

    • Additional information

  • Transcription initiation by SigL-containing [SW|RNA polymerase] requires the activity of ATP-hydrolyzing transcription activators.

    • Expression and Regulation

    Operons

    genes
    [gene|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, transcriptional roadblock [Pubmed|16166551], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|16166551]
    • view in new tab

    Biological materials

    Mutant

  • GP146 ( ''sigL''::spc), available in [SW|Jörg Stülke]'s lab [Pubmed|17183217]
  • GP2302 ( ''sigL''::tet), available in [SW|Jörg Stülke]'s lab
  • 1A914 ( ''sigL''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]

    • Labs working on this gene/protein

  • [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

    • References

    Reviews

  • 7934866,10894718,21054445,26010401,21906631

    • Original publications

  • 16585774,11274109,1924373,16166551,22900538,26293966,24553251