sigL [2017-04-12 09:01:58]

sigma factor of the RNA polymerase, Sigma-54, Sigma L

Locus: BSU34200

Isoelectric point: 7.79

Molecular weight: 49.54 kDa

Protein length: 436 aa

Gene length: 1308 bp

Function: utilization of arginin, acetoin and fructose, required for cold adaptation

Product: RNA polymerase sigma-54 factor (sigma-L)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → Sigma factors
• Information processing → Regulation of gene expression → Sigma factors and their control → Sigma factors

This gene is a member of the following regulons

• CcpA regulon

Gene

Coordinates: 3,512,498 -> 3,513,808

Phenotypes of a mutant

• The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

The protein

Catalyzed reaction/ biological activity

• Binding to promoters of the -12, -24 type

Protein family

• sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family

Domains

• DNA binding domain (H-T-H motif) (324–343)
• pron box domain (413–421)
• 3 x Compositional bias domain (6–21),(32–53),(112–136)

Structure

• 5BYH (E. coli RNA polymerase containing Sigma-54) PubMed

Additional information

• Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.

Expression and Regulation

Operons

• Genes: sigL
  Description: PubMed

  Regulatory mechanism

  • CcpA: repression, transcriptional roadblock PubMed, in CcpA regulon

  Regulation

  • repressed by glucose (CcpA) PubMed

Biological materials

Mutant

• GP146 ( sigL::spc), available in Jörg Stülke's lab PubMed
• GP2302 ( sigL::tet), available in Jörg Stülke's lab
• 1A914 ( sigL::kan), PubMed, available at BGSC

Labs working on this gene/protein

• Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

Reviews

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Original publications

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